EMDB EMD-4909

Single particle reconstruction
4.0Å resolution

E. coli DNA Gyrase - DNA binding and cleavage domain in State 1 without TOPRIM insertion

Map released:
Last modified:
Overview of EMD-4909
Fitted atomic model: 6rks
Related EM entries by publication: EMD-4910, EMD-4912, EMD-4913, EMD-4914, EMD-4915
Primary publication:
Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex.
Vanden Broeck A, Lotz C, Ortiz J, Lamour V
Nat Commun 10 4935-4935 (2019)
PMID: 31666516

Function and Biology Details

Sample name: DNA binding and cleavage domain of the E. coli DNA Gyrase in State 1
Ligand: (3~{R})-3-[[4-(3,4-dihydro-2~{H}-pyrano[2,3-c]pyridin-6-ylmethylamino)piperidin-1-yl]methyl]-1,4,7-triazatricyclo[6.3.1.0^{4,12}]dodeca-6,8(12),9-triene-5,11-dione
Nucleic acids: DNA Strand 1, DNA Strand 2
Proteins: DNA binding and cleavage domain of the E. coli DNA Gyrase in State 1, DNA binding and cleavage domain of the E. coli DNA Gyrase in State 1, DNA binding and cleavage domain of the E. coli DNA Gyrase in State 1, DNA binding and cleavage domain of the E. coli DNA Gyrase in State 1, DNA gyrase subunit A, DNA gyrase subunit B

Experimental Information Details

Resolution: 4.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)