EMDB EMD-4788

Single particle reconstruction
4.46Å resolution

D. melanogaster CMG-DNA with ATP, State 2B

Map released:
Overview of EMD-4788
Source organism: Drosophila melanogaster [7227]
Fitted atomic model: 6raz
Related EM entries by publication: EMD-4785, EMD-4786, EMD-4787
Primary publication:
Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic Replisome.
Eickhoff P, Kose HB, Martino F, Petojevic T, Abid Ali F, Locke J, Tamberg N, Nans A, Berger JM, Botchan MR, Yardimci H, Costa A
Cell Rep 28 2673-2688.e8 (2019)
PMID: 31484077

Function and Biology Details

Sample name: CMG helicase bound to forked DNA in the presence of ATP, State 2B
Ligands: ADENOSINE-5'-DIPHOSPHATE, ADENOSINE-5'-TRIPHOSPHATE
Nucleic acids: DNA, DNA
Proteins: CMG helicase bound to forked DNA in the presence of ATP, State 2B, CMG helicase, DNA, CDC45L, IP07275p, Probable DNA replication complex GINS protein PSF2, AT18545p, DNA replication complex GINS protein SLD5, DNA replication licensing factor Mcm2, DNA replication licensing factor Mcm5, DNA replication licensing factor Mcm6, DNA replication licensing factor Mcm3, DNA replication licensing factor MCM4, DNA replication licensing factor Mcm7

Experimental Information Details

Resolution: 4.46Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)