EMDB EMD-4787

Single particle reconstruction
4.28Å resolution

D. melanogaster CMG-DNA with ATP, State 2A

Map released:
Last modified:
Overview of EMD-4787
Source organism: Drosophila melanogaster [7227]
Fitted atomic model: 6ray
Related EM entries by publication: EMD-4785, EMD-4786, EMD-4788
Primary publication:
Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic Replisome.
Eickhoff P, Kose HB, Martino F, Petojevic T, Abid Ali F, Locke J, Tamberg N, Nans A, Berger JM, Botchan MR, Yardimci H, Costa A
Cell Rep 28 2673-2688.e8 (2019)
PMID: 31484077

Function and Biology Details

Sample name: CMG helicase bound to forked DNA in the presence of ATP, State 2A
Ligands: ADENOSINE-5'-TRIPHOSPHATE, ADENOSINE-5'-DIPHOSPHATE
Nucleic acid: DNA (5'-D(P*AP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3')
Proteins: CMG helicase bound to forked DNA in the presence of ATP, State 2A, CMG helicase, DNA, DNA replication licensing factor Mcm3, DNA replication licensing factor MCM4, DNA replication licensing factor Mcm7, DNA replication licensing factor Mcm6, DNA replication licensing factor Mcm5, CDC45L, IP07275p, Probable DNA replication complex GINS protein PSF2, AT18545p, DNA replication complex GINS protein SLD5, DNA replication licensing factor Mcm2

Experimental Information Details

Resolution: 4.28Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)