Single particle reconstruction
3.99Å resolution

D. melanogaster CMG-DNA with ATP, State 1B

Map released:
Last modified:
Overview of EMD-4786
Source organism: Drosophila melanogaster [7227]
Fitted atomic model: 6rax
Related EM entries by publication: EMD-4785, EMD-4787, EMD-4788
Primary publication:
Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic Replisome.
Eickhoff P, Kose HB, Martino F, Petojevic T, Abid Ali F, Locke J, Tamberg N, Nans A, Berger JM, Botchan MR, Yardimci H, Costa A
Cell Rep 28 2673-2688.e8 (2019)
PMID: 31484077

Function and Biology Details

Sample name: CMG helicase bound to forked DNA in the presence of ATP, State 1B
Nucleic acids: DNA, DNA
Proteins: CMG helicase bound to forked DNA in the presence of ATP, State 1B, CMG helicase, DNA, DNA replication licensing factor Mcm2, DNA replication licensing factor Mcm3, DNA replication licensing factor MCM4, DNA replication licensing factor Mcm5, DNA replication licensing factor Mcm6, DNA replication licensing factor Mcm7, CDC45L, IP07275p, Probable DNA replication complex GINS protein PSF2, AT18545p, DNA replication complex GINS protein SLD5

Experimental Information Details

Resolution: 3.99Å
Resolution method: FSC 0.143 CUT-OFF
Detector: GATAN K2 SUMMIT (4k x 4k)