EMDB EMD-4768

Single particle reconstruction
3.5Å resolution

Cryo-EM structure of NCP_THF2(-3)

Map released:
Overview of EMD-4768
Source organism: Homo sapiens [9606]
Fitted atomic model: 6r94
Related EM entries by publication: EMD-4762, EMD-4763, EMD-4764, EMD-4765, EMD-4766, EMD-4767
Primary publication:
DNA damage detection in nucleosomes involves DNA register shifting.
Matsumoto S, Cavadini S, Bunker RD, Grand RS, Potenza A, Rabl J, Yamamoto J, Schenk AD, Schubeler D, Iwai S, Sugasawa K, Kurumizaka H, Thoma NH
Nature - (2019)
PMID: 31142837

Function and Biology Details

Sample name: THF2 (-3) containing nucleosome
Nucleic acids: Human alpha-satellite DNA (145-MER), Human alpha-satellite DNA (145-MER) with abasic sites at positions 97-98
Proteins: THF2 (-3) containing nucleosome, Human DNA, Histone H4, Histone H3.1 / 2A / 2B, Histone H3.1, Histone H4, Histone H2A type 1-B/E, Histone H2B type 1-J

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)