EMDB EMD-4767

Single particle reconstruction
4.0Å resolution

Cryo-EM structure of NCP-6-4PP

Map released:
Overview of EMD-4767
Source organism: Homo sapiens [9606]
Fitted atomic model: 6r93
Related EM entries by publication: EMD-4762, EMD-4763, EMD-4764, EMD-4765, EMD-4766, EMD-4768
Primary publication:
DNA damage detection in nucleosomes involves DNA register shifting.
Matsumoto S, Cavadini S, Bunker RD, Grand RS, Potenza A, Rabl J, Yamamoto J, Schenk AD, Schubeler D, Iwai S, Sugasawa K, Kurumizaka H, Thoma NH
Nature - (2019)
PMID: 31142837

Function and Biology Details

Sample name: 6-4PP nucleosome
Nucleic acids: Human alpha-satellite DNA (145-MER), Human alpha-satellite DNA (145-MER) with a 6-4PP at positions 95-96
Proteins: 6-4PP nucleosome, DNA, Histone H3.1, Histone H2A, Histone H2B, Histone H4, Histone H3.1, Histone H4, Histone H2A type 1-B/E, Histone H2B type 1-J

Experimental Information Details

Resolution: 4.0Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)