EMDB EMD-4765

Single particle reconstruction
4.1Å resolution

Cryo-EM structure of NCP_THF2(-3)-UV-DDB

Map released:
Overview of EMD-4765
Source organism: Homo sapiens [9606]
Fitted atomic model: 6r91
Related EM entries by publication: EMD-4762, EMD-4763, EMD-4764, EMD-4766, EMD-4767, EMD-4768
Primary publication:
DNA damage detection in nucleosomes involves DNA register shifting.
Matsumoto S, Cavadini S, Bunker RD, Grand RS, Potenza A, Rabl J, Yamamoto J, Schenk AD, Schubeler D, Iwai S, Sugasawa K, Kurumizaka H, Thoma NH
Nature - (2019)
PMID: 31142837

Function and Biology Details

Sample name: UV-DDB bound to a THF2 (-3) containing nucleosome
Nucleic acids: Human alpha-satellite DNA (145-MER), Human alpha-satellite DNA (145-MER) with abasic sites at positions 97-98
Proteins: UV-DDB bound to a THF2 (-3) containing nucleosome, Histone H3.1, Histone H2A, Histone H2B, Histone H4, DNA, DNA damage-binding protein 1(2), DNA damage-binding protein 2, Histone H3.1, Histone H4, Histone H2A type 1-B/E, Histone H2B type 1-J, DNA damage-binding protein 1, DNA damage-binding protein 2

Experimental Information Details

Resolution: 4.1Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)