EMDB EMD-4762

Single particle reconstruction
4.3Å resolution

Cryo-EM structure of NCP-6-4PP(-1)-UV-DDB

Map released:
Overview of EMD-4762
Source organism: Homo sapiens [9606]
Fitted atomic model: 6r8y
Related EM entries by publication: EMD-4763, EMD-4764, EMD-4765, EMD-4766, EMD-4767, EMD-4768
Primary publication:
DNA damage detection in nucleosomes involves DNA register shifting.
Matsumoto S, Cavadini S, Bunker RD, Grand RS, Potenza A, Rabl J, Yamamoto J, Schenk AD, Schubeler D, Iwai S, Sugasawa K, Kurumizaka H, Thoma NH
Nature - (2019)
PMID: 31142837

Function and Biology Details

Sample name: UV-DDB bound to a 6-4PP containing nucleosome
Nucleic acids: Human alpha-satellite DNA (145-MER), Human alpha-satellite DNA (145-MER) with a 6-4PP at positions 95-96
Proteins: UV-DDB bound to a 6-4PP containing nucleosome, histones, DNA damage-binding proteins, DNA, histones, Histone H3.1, Histone H4, Histone H2A type 1-B/E, Histone H2B type 1-J, Histone H4, DNA damage-binding protein 1, DNA damage-binding protein 2

Experimental Information Details

Resolution: 4.3Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)