EMDB EMD-4743

Single particle reconstruction
6.0Å resolution

Ribosome-bound SecYEG translocon in a nanodisc

Map released:
Last modified:
Overview of EMD-4743
Source organism: Escherichia coli [562]
Fitted atomic model: 6r7l
Primary publication:
Partially inserted nascent chain unzips the lateral gate of the Sec translocon.
Kater L, Frieg B, Berninghausen O, Gohlke H, Beckmann R, Kedrov A
Embo Rep. e48191-e48191 (2019)
PMID: 31379073

Function and Biology Details

Sample name: Stalled E. coli ribosome nascent chain complex (RNC) bound to the translocon SecYEG in a nanodisc
Proteins: Stalled E. coli ribosome nascent chain complex (RNC) bound to the translocon SecYEG in a nanodisc, SecG, SecE,Protein translocase subunit SecE,Protein translocase subunit SecE, Protein translocase subunit SecY

Experimental Information Details

Resolution: 6.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)