Single particle reconstruction
3.7Å resolution

Structure of XBP1u-paused ribosome nascent chain complex with SRP.

Map released:
Overview of EMD-4735
Source organisms:
Fitted atomic model: 6r6g
Related EM entries by publication: EMD-4729, EMD-4737, EMD-4745
Primary publication:
Structural and mutational analysis of the ribosome-arresting human XBP1u.
Shanmuganathan V, Schiller N, Magoulopoulou A, Cheng J, Braunger K, Cymer F, Berninghausen O, Beatrix B, Kohno K, Heijne GV, Beckmann R
Elife 8 - (2019)
PMID: 31246176

Function and Biology Details

Sample name: Structure of XBP1u-paused ribosome nascent chain complex with SRP.
Nucleic acids: P-tRNA, E-tRNA, mRNA, 28S ribosomal RNA, 5S ribosomal RNA, 5.8S ribosomal RNA, 18S ribosomal RNA, 7S RNA
Proteins: Structure of XBP1u-paused ribosome nascent chain complex with SRP., Structure of XBP1u-paused nascent chain complex with SRP., P- and E- site tRNA, X-box-binding protein 1, mRNA encoding XBP1u, SRP, Signal sequence (HR2), eS31, X-box-binding protein 1, ribosomal protein RACK1, uS14, uL2, 40S ribosomal protein S30, uL3, 40S ribosomal protein S7, uL4, 40S ribosomal protein S8, Ribosomal protein S9 (Predicted), 60S ribosomal protein L5, Ribosomal protein S11, 60S ribosomal protein L6, Ribosomal protein S28, uL30, Ribosomal protein S20, eL8, uL6, 40S ribosomal protein S21, Ribosomal protein L10 (Predicted), uS13, 40S ribosomal protein S27, Ribosomal protein L11, eS17, 60S ribosomal protein L13, 40S ribosomal protein S26, Ribosomal protein L14, 40S ribosomal protein S14, Ribosomal protein L15, 40S ribosomal protein S24, 60S ribosomal protein L13a, ribosomal protein eS25, uL22, eS19, eL18, ribosomal protein uS15, 60S ribosomal protein L19, 40S ribosomal protein S12, eL20, eS10, eL21, Ribosomal protein S15a, Ribosomal protein S16, 60S ribosomal protein L22, Ribosomal protein S23, eL14, Ribosomal protein L24, uS19, uL23, Ribosomal protein L26, 60S ribosomal protein L27, uL15, eL29, eL30, eL31, eL32, eL33, eL34, uL29, 60S ribosomal protein L36, Ribosomal protein L37, 60S ribosomal protein L38, eL39, 60S ribosomal protein L40, 60s ribosomal protein l41, eL42, ribosomal protein eL43, uS2, eL28, 60S acidic ribosomal protein P0, uL11, 40S ribosomal protein S3a, uS5, Ribosomal protein S3, 40S ribosomal protein S4, Ribosomal protein S5, 40S ribosomal protein S6, Signal recognition particle 54 kDa protein, Signal recognition particle subunit SRP19, Signal recognition particle subunit SRP68, Signal recognition particle 9 kDa protein, Signal recognition particle 14 kDa protein, Signal sequence (HR2)

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Detector: FEI FALCON II (4k x 4k)