Single particle reconstruction
3.0Å resolution

Structure of XBP1u-paused ribosome nascent chain complex (post-state)

Map released:
Overview of EMD-4729
Source organisms:
Fitted atomic model: 6r5q
Related EM entries by publication: EMD-4735, EMD-4737, EMD-4745
Primary publication:
Structural and mutational analysis of the ribosome-arresting human XBP1u.
Shanmuganathan V, Schiller N, Magoulopoulou A, Cheng J, Braunger K, Cymer F, Berninghausen O, Beatrix B, Kohno K, Heijne GV, Beckmann R
Elife 8 - (2019)
PMID: 31246176

Function and Biology Details

Sample name: Structure of XBP1u-paused ribosome nascent chain complex (post-state).
Nucleic acids: E-tRNA, P-tRNA, 28S ribosomal RNA, 5S rRNA, 5.8S ribosomal RNA, 18S ribosomal RNA, mRNA
Proteins: Structure of XBP1u-paused ribosome nascent chain complex (post-state)., ribosome, tRNA, X-box-binding protein 1, mRNA, X-box-binding protein 1, uL2, uL3, uL4, 60S ribosomal protein L5, 60S ribosomal protein L6, uL30, eL8, uL6, Ribosomal protein L10 (Predicted), Ribosomal protein L11, 60S ribosomal protein L13, Ribosomal protein L14, Ribosomal protein L15, uL13, uL22, eL18, eL19, eL20, eL21, eL22, eL14, Ribosomal protein L24, uL23, Ribosomal protein L26, 60S ribosomal protein L27, uL15, eL29, eL30, eL31, eL32, eL33, eL34, uL29, 60S ribosomal protein L36, Ribosomal protein L37, eL38, eL39, eL40, 60s ribosomal protein l41, eL42, ribosomal protein eL43, eL28, 60S acidic ribosomal protein P0, uL11, uS2, 40S ribosomal protein S3a, uS5, Ribosomal protein S3, 40S ribosomal protein S4, Ribosomal protein S5, 40S ribosomal protein S6, 40S ribosomal protein S7, 40S ribosomal protein S8, Ribosomal protein S9 (Predicted), eS10, Ribosomal protein S11, 40S ribosomal protein S12, ribosomal protein uS15, uS11, uS17, Ribosomal protein S16, eS17, uS13, eS19, uS10, eS21, Ribosomal protein S15a, Ribosomal protein S23, eS24, ribosomal protein eS25, eS26, 40S ribosomal protein S27, Ribosomal protein S28, uS14, eS31, ribosomal protein RACK1, 40S ribosomal protein S30

Experimental Information Details

Resolution: 3.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Detector: FEI FALCON II (4k x 4k)