EMDB EMD-4638

Single particle reconstruction
3.0Å resolution

Structure of a bacterial 50S ribosomal subunit in complex with the novel quinoxolidinone antibiotic cadazolid

Map released:
Last modified:
Overview of EMD-4638
Source organism: Escherichia coli [562]
Fitted atomic model: 6qul
Related EM entry by publication: EMD-4639, EMD-4641
Primary publication:
Structural basis of translation inhibition by cadazolid, a novel quinoxolidinone antibiotic.
Scaiola A, Leibundgut M, Boehringer D, Caspers P, Bur D, Locher HH, Rueedi G, Ritz D
Sci Rep 9 5634-5634 (2019)
PMID: 30948752

Function and Biology Details

Sample name: Structure of a bacterial 50S ribosomal subunit in complex with the novel quinoxolidinone antibiotic cadazolid
Ligands: N-FORMYLMETHIONINE, MAGNESIUM ION, ADENOSINE-5'-MONOPHOSPHATE, cadazolid, ZINC ION
Nucleic acids: P-site fMet-tRNA(fMet), 23S rRNA, 5S rRNA
Proteins: Structure of a bacterial 50S ribosomal subunit in complex with the novel quinoxolidinone antibiotic cadazolid, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36

Experimental Information Details

Resolution: 3.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)