EMDB EMD-4631

Single particle reconstruction
24.8Å resolution

A dimer component of alpha-1 antitrypsin polymers isolated from ZZ explant liver tissue and decorated with Fab 4B12 (component B with ~90 degree rotation around the dimer axis)

Map released:
Overview of EMD-4631
Source organisms:
To be published

Function and Biology Details

Sample name: Polymers of the severe deficiency Z variant of alpha-1 antitrypsin labelled with 4B12 Fab fragments
Cellular component: Polymers of the severe deficiency Z variant of alpha-1 antitrypsin labelled with 4B12 Fab fragments
Proteins: Complex between two 4B12 Fab fragments and a dimer component of polymers of the severe deficiency Z alpha-1 antitrypsin variant, Alpha-1 antitrypsin, 4B12 Fab heavy chain, 4B12 Fab light chain

Experimental Information Details

Resolution: 24.8Å
Resolution method: FSC 0.33 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION, RELION
Microscope: FEI TECNAI F20
Detector: DIRECT ELECTRON DE-20 (5k x 3k)