EMDB EMD-4630

Single particle reconstruction
3.4Å resolution

Cryo-EM structures of Lsg1-TAP pre-60S ribosomal particles

Map released:
Last modified:
Overview of EMD-4630
Source organism: Baker's yeast [4932]
Fitted atomic model: 6qt0
Primary publication:
Mechanism of completion of peptidyltransferase centre assembly in eukaryotes.
Kargas V, Castro-Hartmann P, Escudero-Urquijo N, Dent K, Hilcenko C, Sailer C, Zisser G, Marques-Carvalho MJ, Pellegrino S, Wawiorka L, Freund SM, Wagstaff JL, Andreeva A, Faille A, Chen E, Stengel F, Bergler H, Warren AJ
Elife 8 - (2019)
PMID: 31115337

Function and Biology Details

Sample name: Lsg1-TAP 60s ribosomal subunit
Ligand: ZINC ION
Nucleic acids: 25S rRNA, 5S rRNA, 5.8S rRNA
Proteins: Lsg1-TAP 60s ribosomal subunit, 60S ribosomal protein L2-A, 60S ribosomal protein L3, 60S ribosomal protein L4-A, 60S ribosomal protein L11-A, 60S ribosomal protein L9-A, 60S ribosomal protein L6-A, 60S ribosomal protein L8-A, 60S ribosomal protein L10, 60S ribosomal protein L16-B, 60S ribosomal protein L13-A, 60S ribosomal protein L23-A, 60S ribosomal protein L14-A, 60S ribosomal protein L28, 60S ribosomal protein L15-A, 60S ribosomal protein L5, 60S ribosomal protein L18-A, 60S ribosomal protein L19-A, 60S ribosomal protein L20-A, 60S ribosomal protein L21-A, 60S ribosomal protein L17-A, 60S ribosomal protein L22-A, 60S ribosomal protein L25, 60S ribosomal protein L26-A, 60S ribosomal protein L27-A, 60S ribosomal protein L35-A, 60S ribosomal protein L29, 60S ribosomal protein L7-A, 60S ribosomal protein L30, 60S ribosomal protein L31-A, 60S ribosomal protein L32, 60S ribosomal protein L33-A, 60S ribosomal protein L34-A, 60S ribosomal protein L36-A, 60S ribosomal protein L37-A, 60S ribosomal protein L38, 60S ribosomal protein L39, 60S ribosomal protein L42-A, 60S ribosomal protein L43-A, Eukaryotic translation initiation factor 6, Ubiquitin-60S ribosomal protein L40, 60S ribosomal protein L24-A, Cytoplasmic 60S subunit biogenesis factor REH1

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)