EMDB EMD-4598

Single particle reconstruction
4.38Å resolution

Rhodopsin-Gi protein complex

Map released:
Overview of EMD-4598
Source organisms:
Fitted atomic model: 6qno
Primary publication:
Cryo-EM structure of the rhodopsin-G alpha i-beta gamma complex reveals binding of the rhodopsin C-terminal tail to the G beta subunit.
Tsai CJ, Marino J, Adaixo R, Pamula F, Muehle J, Maeda S, Flock T, Taylor NM, Mohammed I, Matile H, Dawson RJ, Deupi X, Stahlberg H, Schertler G
Elife 8 - (2019)
PMID: 31251171

Function and Biology Details

Sample name: Rhodopsin-Gi complex bound with antibody fragment Fab16
Ligands: RETINAL, N-ACETYL-D-GLUCOSAMINE
Proteins: Rhodopsin-Gi complex bound with antibody fragment Fab16, Guanine nucleotide-binding protein alpha subunit, Guanine nucleotide-binding protein beta/gamma subunit, antibody FAB fragment Fab16, Rhodopsin, Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(T) subunit gamma-T1, Fab antibody fragment light chain, Fab antibody fragment heavy chain, Rhodopsin

Experimental Information Details

Resolution: 4.38Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)