EMD-45632

Single-particle
3.4 Å
EMD-45632
3D View Gallery
Deposition: 06/07/2024
Map released: 08/01/2025
Last modified: 18/06/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-45632
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9cji summary report (pdf.gz) Map and 9cji FULL report (pdf.gz)
EMICSS annotations (xml)

Cas12a:gRNA:DNA (Acidaminococcus sp.) with 0 RNA:DNA base pairs, structure 2

EMD-45632

Single-particle
3.4 Å
EMD-45632
3D View Gallery
Deposition: 06/07/2024
Map released: 08/01/2025
Last modified: 18/06/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Acidaminococcus sp. BV3L6
Sample: Cas12a:gRNA:DNA (Acidaminococcus sp.) with 0 RNA:DNA base pairs, structure 2
Fitted models: 9cji

Deposition Authors: Soczek KM, Doudna JA
CRISPR-Cas12a bends DNA to destabilize base pairs during target interrogation.
Soczek KM, Cofsky JC, Tuck OT, Shi H , Doudna JA
(2025) Nucleic Acids Res , 53
PUBMED: 39698811
DOI: doi:10.1093/nar/gkae1192
ISSN: 1362-4962
ASTM: NARHAD