EMD-45586

Single-particle
3.0 Å
EMD-45586
3D View Gallery
Deposition: 01/07/2024
Map released: 04/12/2024
Last modified: 22/01/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-45586

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9cgu summary report (pdf.gz) Map-9cgu FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cas9 ternary complex, 14-nt sgRNA, State II (kinked)

EMD-45586

Single-particle
3.0 Å
EMD-45586
3D View Gallery
Deposition: 01/07/2024
Map released: 04/12/2024
Last modified: 22/01/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Streptococcus pyogenes
Sample: Cas9-sgRNA-dsDNA complex
Fitted models: 9cgu

Deposition Authors: Kiernan KA , Simonovic M
Structural basis of Cas9 DNA interrogation with a 5' truncated sgRNA.
Kiernan KA , Kwon J, Merrill BJ, Simonovic M
(2025) Nucleic Acids Res , 53
PUBMED: 39657754
DOI: doi:10.1093/nar/gkae1164
ISSN: 1362-4962
ASTM: NARHAD