EMDB EMD-4527

Single particle reconstruction
3.0Å resolution

Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, focused refinement on core

Map released:
Overview of EMD-4527
Source organism: Homo sapiens [9606]
Primary publication:
A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation.
Fica SM, Oubridge C, Wilkinson ME, Newman AJ, Nagai K
Science 363 710-714 (2019)
PMID: 30705154

Function and Biology Details

Sample name: Human post-catalytic P complex spliceosome
Protein: Human post-catalytic P complex spliceosome

Experimental Information Details

Resolution: 3.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)