EMDB EMD-4476

Single particle reconstruction
3.9Å resolution

Structure of tmRNA SmpB bound in A site of E. coli 70S ribosome

Map released:
Overview of EMD-4476
Source organism: Escherichia coli [562]
Fitted atomic model: 6q97
Related EM entry by publication: EMD-4475, EMD-4477
Primary publication:
How a circularized tmRNA moves through the ribosome.
Rae CD, Gordiyenko Y, Ramakrishnan V
Science 363 740-744 (2019)
PMID: 30765567

Function and Biology Details

Sample name: tmRNA SmpB bound in A site of E. coli 70S ribosome
Cellular component: 70S ribosome
Ligands: MAGNESIUM ION, ZINC ION, water
Nucleic acids: 23S ribosomal RNA, 16S ribosomal RNA, 5S ribosomal RNA, tRNA-Met, mRNA, transfer-messenger RNA (tmRNA), tRNA-Val
Proteins: tmRNA SmpB bound in A site of E. coli 70S ribosome, tmRNA-SmpB, nascent peptide, mRNA, P-site tRNA, E-site tRNA, SsrA-binding protein, Nascent peptide, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L10, 50S ribosomal protein L11, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L31, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, 30S ribosomal protein S2, 30S ribosomal protein S3, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, 30S ribosomal protein S18, 30S ribosomal protein S19, 30S ribosomal protein S20, 30S ribosomal protein S21

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)