EMDB EMD-4474

Single particle reconstruction
3.1Å resolution

Cryo-EM structure of the mRNA translating and degrading yeast 80S ribosome-Xrn1 nuclease complex

Map released:
Overview of EMD-4474
Source organism: Baker's yeast [4932]
Fitted atomic model: 6q8y
Primary publication:
Structure of the 80S ribosome-Xrn1 nuclease complex
Tesina P, Heckel E, Cheng J, Buschauer R, Kater L, Berninghausen O, Fromont-Racine M, Beatrix B, Jacquier A, Becker T, Beckmann R
Nat.Struct.Mol.Biol. - (2019)

Function and Biology Details

Sample name: Yeast 80S ribosome in complex with the Xrn11 exoribonuclease obtained from natural source.
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: 18S ribosomal RNA, mRNA, P-site tRNA, E-site tRNA, 25S ribosomal RNA, 5S ribosomal RNA, 5.8S ribosomal RNA
Proteins: Yeast 80S ribosome in complex with the Xrn11 exoribonuclease obtained from natural source., ribosome, mRNA, 5'-3' exoribonuclease Xrn1, P-site tRNA, E-site tRNA, 60S ribosomal protein L8-A, 60S ribosomal protein L23-A, 60S ribosomal protein L36-A, 60S ribosomal protein L9-A, 60S ribosomal protein L24-A, 60S ribosomal protein L37-A, 60S ribosomal protein L11-B, 60S ribosomal protein L25, 60S ribosomal protein L38, 60S ribosomal protein L13-A, 60S ribosomal protein L26-A, 60S ribosomal protein L39, 60S ribosomal protein L14-A, 60S ribosomal protein L27-A, Ubiquitin-60S ribosomal protein L40, 60S ribosomal protein L42-A, 60S ribosomal protein L15-A, 60S ribosomal protein L28, 60S ribosomal protein L41-B, 60S ribosomal protein L43-A, 60S ribosomal protein L16-A, 60S ribosomal protein L29, 60S ribosomal protein L2-A, 60S ribosomal protein L17-A, 40S ribosomal protein S21-A, 40S ribosomal protein S22-A, 40S ribosomal protein S23-A, 40S ribosomal protein S24-A, 40S ribosomal protein S26-B, 40S ribosomal protein S27-A, 40S ribosomal protein S30-A, 5'-3' exoribonuclease 1, 60S ribosomal protein L30, 60S ribosomal protein L3, 60S ribosomal protein L18-A, 60S ribosomal protein L31-A, 60S ribosomal protein L10, 60S ribosomal protein L4-A, 60S ribosomal protein L19-A, 60S ribosomal protein L32, 60S ribosomal protein L20-A, 60S ribosomal protein L5, 60S ribosomal protein L21-A, 60S ribosomal protein L33-A, 60S ribosomal protein L22-A, 60S ribosomal protein L6-A, 60S ribosomal protein L34-A, 60S ribosomal protein L7-A, 60S ribosomal protein L35-A, 40S ribosomal protein S3, Rps5p, 40S ribosomal protein S10-A, 40S ribosomal protein S12, 40S ribosomal protein S15, 40S ribosomal protein S16-A, 40S ribosomal protein S17-A, 40S ribosomal protein S18-A, 40S ribosomal protein S20, 40S ribosomal protein S25-A, 40S ribosomal protein S28-A, 40S ribosomal protein S29-A, Ubiquitin-40S ribosomal protein S31, Guanine nucleotide-binding protein subunit beta-like protein, 40S ribosomal protein S0-A, 40S ribosomal protein S1-A, 40S ribosomal protein S2, 40S ribosomal protein S4-A, 40S ribosomal protein S6-A, 40S ribosomal protein S7-A, 40S ribosomal protein S8-A, 40S ribosomal protein S9-A, 40S ribosomal protein S11-A, 40S ribosomal protein S13, 40S ribosomal protein S14-B, 40S ribosomal protein S19-A

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)