EMDB EMD-4467

Single particle reconstruction
3.7Å resolution

Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2delD1-Cks2 complex (with the D1 box mutated)

Map released:
Overview of EMD-4467
Source organism: Homo sapiens [9606]
Related EM entries by publication: EMD-4463, EMD-4464, EMD-4465, EMD-4466
Primary publication:
Cyclin A2 degradation during the spindle assembly checkpoint requires multiple binding modes to the APC/C.
Zhang S, Tischer T, Barford D
Nat Commun 10 3863-3863 (2019)
PMID: 31455778

Function and Biology Details

Sample name: APC/C-Cdc20-Cdk2-cyclinA2delD1-Cks2 complex (with the D1 box mutated)
Proteins: APC/C-Cdc20-Cdk2-cyclinA2delD1-Cks2 complex (with the D1 box mutated), Apc1, Apc2, Apc3, Apc4, Apc5, Apc6, Apc7, Apc8, Apc10, Apc11, Apc12, Apc13, Apc15, Apc16, Cdc20, Cdk2, Cyclin A2, Cks2

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)