EMDB EMD-4465

Single particle reconstruction
3.2Å resolution

Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D1 box class

Map released:
Overview of EMD-4465
Source organism: Homo sapiens [9606]
Fitted atomic model: 6q6g
Related EM entries by publication: EMD-4463, EMD-4464, EMD-4466, EMD-4467
Primary publication:
Cyclin A2 degradation during the spindle assembly checkpoint requires multiple binding modes to the APC/C.
Zhang S, Tischer T, Barford D
Nat Commun 10 3863-3863 (2019)
PMID: 31455778

Function and Biology Details

Sample name: Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D1 box class
Proteins: Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D1 box class, Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D1 box class, Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D1 box class, Cell division cycle protein 20 homolog, Cyclin-A2, Anaphase-promoting complex subunit 10, Anaphase-promoting complex subunit 15, Anaphase-promoting complex subunit 1,Anaphase-promoting complex subunit 1, Anaphase-promoting complex subunit 2, Anaphase-promoting complex subunit 4, Anaphase-promoting complex subunit 5, Cell division cycle protein 16 homolog, Anaphase-promoting complex subunit 11, Anaphase-promoting complex subunit CDC26, Anaphase-promoting complex subunit 13, Anaphase-promoting complex subunit 16, Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 7, Cell division cycle protein 23 homolog

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)