EMDB EMD-4463

Single particle reconstruction
3.5Å resolution

Cryo-EM reconstruction of the APC/C-MCC-Cdk2-cyclinA2-Cks2 complex in the open conformation

Map released:
Overview of EMD-4463
Source organism: Homo sapiens [9606]
Related EM entries by publication: EMD-4464, EMD-4465, EMD-4466, EMD-4467
Primary publication:
Cyclin A2 degradation during the spindle assembly checkpoint requires multiple binding modes to the APC/C.
Zhang S, Tischer T, Barford D
Nat Commun 10 3863-3863 (2019)
PMID: 31455778

Function and Biology Details

Sample name: Cryo-EM reconstruction of the APC/C-MCC-Cdk2-cyclinA2-Cks2 complex in the open conformation
Proteins: Cryo-EM reconstruction of the APC/C-MCC-Cdk2-cyclinA2-Cks2 complex in the open conformation, Apc1, Apc2, Apc3, Apc4, Apc5, Apc6, Apc7, Apc8, Apc10, Apc11, Apc12, Apc13, Apc15, Apc16, Cdc20, Cdk2, Cyclin A2, Cks2, BubR1, Mad2, Bub3

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI POLARA 300
Detector: FEI FALCON III (4k x 4k)