EMDB EMD-4370

Single particle reconstruction
3.2Å resolution

Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit.

Map released:
Overview of EMD-4370
Source organisms:
Fitted atomic model: 6gb2
Related EM entry by publication: EMD-4369
Primary publication:
Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM
Kummer E, Leibundgut M, Rackham O, Lee RG, Boehringer D, Filipovska A, Ban N
Nature - (2018)

Function and Biology Details

Sample name: mammalian mitochondrial translation initiation complex
Ligands: MAGNESIUM ION, ZINC ION, GUANOSINE-5'-MONOPHOSPHATE, SPERMINE, 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE, SODIUM ION, N-FORMYLMETHIONINE, water
Nucleic acids: 16S ribosomal RNA, mitochondrial, CP tRNAPhe, mitochondrial, P-site fMet-tRNAMet, mitochondrial
Proteins: mammalian mitochondrial translation initiation complex, mammalian mitochondrial ribosome, Translation initiation factor IF-2, mitochondrial, tRNA, Mitochondrial ribosomal protein L12, Mitochondrial ribosomal protein L27, Mitochondrial ribosomal protein L28, Mitochondrial ribosomal protein L47, 'Mitochondrial ribosomal protein L30, 'Mitochondrial ribosomal protein L55, Mitochondrial ribosomal protein L32, Mitochondrial ribosomal protein L33, Mitochondrial ribosomal protein L34, Mitochondrial ribosomal protein L35, Ribosomal protein, Translation initiation factor IF-2, mitochondrial, Mitochondrial ribosomal protein L2, ICT1, Mitochondrial ribosomal protein L4, Mitochondrial ribosomal protein L9, Mitochondrial ribosomal protein L10, Mitochondrial ribosomal protein L11, Mitochondrial ribosomal protein L13, Mitochondrial ribosomal protein L14, Mitochondrial ribosomal protein L15, Mitochondrial ribosomal protein L16, Mitochondrial ribosomal protein L17, Mitochondrial ribosomal protein L18, Mitochondrial ribosomal protein L19, Mitochondrial ribosomal protein L20, Mitochondrial ribosomal protein L21, Mitochondrial ribosomal protein L22, Mitochondrial ribosomal protein L23, Mitochondrial ribosomal protein L24, Mitochondrial ribosomal protein L37, Mitochondrial ribosomal protein L38, Mitochondrial ribosomal protein L39, Mitochondrial ribosomal protein L40, Mitochondrial ribosomal protein L41, Mitochondrial ribosomal protein L42, Mitochondrial ribosomal protein L43, Mitochondrial ribosomal protein L44, Mitochondrial ribosomal protein L45, Mitochondrial ribosomal protein L46, Mitochondrial ribosomal protein L48, Mrpl34, Mitochondrial ribosomal protein L50, Mitochondrial ribosomal protein L51, Mitochondrial ribosomal protein L52, mL53, MRPL53, Uncharacterized protein, Mitochondrial ribosomal protein L57, Mitochondrial ribosomal protein L58, 'Mitochondrial ribosomal protein L59, mL65, MRPS30, Mitochondrial ribosomal protein S18A, unassigned secondary structure elements

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)