EMDB EMD-4327

Single particle reconstruction
3.5Å resolution

Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (Map C1)

Map released:
Overview of EMD-4327
Fitted atomic model: 6fyx
Related EM entries by publication: EMD-4328, EMD-4329, EMD-4330, EMD-4331
Primary publication:
Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition.
Llacer JL, Hussain T, Saini AK, Nanda JD, Kaur S, Gordiyenko Y, Kumar R, Hinnebusch AG, Lorsch JR, Ramakrishnan V
Elife 7 - (2018)
PMID: 30475211

Function and Biology Details

Sample name: Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)
Ligands: MAGNESIUM ION, ZINC ION, METHIONINE, PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
Nucleic acids: tRNAi, 18S ribosomal RNA, mRNA (31-MER)
Proteins: Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1), Ribosome, tRNA, Initiation factors, Initialtion factors, mRNA, 40S ribosomal protein S0, 40S ribosomal protein S1, KLLA0F09812p, KLLA0D08305p, 40S ribosomal protein S4, KLLA0D10659p, 40S ribosomal protein S6, 40S ribosomal protein S7, 40S ribosomal protein S8, KLLA0E23673p, KLLA0B08173p, KLLA0A10483p, 40S ribosomal protein S12, KLLA0F18040p, 40S ribosomal protein S14, KLLA0F07843p, 40S ribosomal protein S16, KLLA0B01474p, KLLA0B01562p, KLLA0A07194p, KLLA0F25542p, 40S ribosomal protein S21, 40S ribosomal protein S22, KLLA0B11231p, 40S ribosomal protein S24, KLLA0B06182p, 40S ribosomal protein S26, 40S ribosomal protein S27, 40S ribosomal protein S28, 40S ribosomal protein S29, 40S ribosomal protein S30, Ubiquitin-40S ribosomal protein S27a, KLLA0E12277p, 60S ribosomal protein L41-A, Eukaryotic translation initiation factor 1A, Eukaryotic translation initiation factor 2 subunit alpha, Eukaryotic translation initiation factor 2 subunit gamma, Eukaryotic translation initiation factor 2 subunit beta, Eukaryotic translation initiation factor 5, Eukaryotic translation initiation factor 3 subunit A, Eukaryotic translation initiation factor 3 subunit B, eIF3c,eIF3c, Eukaryotic translation initiation factor 3 subunit G, Eukaryotic translation initiation factor 3 subunit I

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI POLARA 300
Detector: FEI FALCON III (4k x 4k)