EMDB EMD-4321

Single particle reconstruction
5.4Å resolution

26S proteasome, s3 state

Map released:
Last modified:
Overview of EMD-4321
Source organism: Baker's yeast [559292]
Fitted atomic model: 6fvv
Related EM entries by publication: EMD-4322, EMD-4323, EMD-4324
Primary publication:
Expanded Coverage of the 26S Proteasome Conformational Landscape Reveals Mechanisms of Peptidase Gating.
Eisele MR, Reed RG, Rudack T, Schweitzer A, Beck F, Nagy I, Pfeifer G, Plitzko JM, Baumeister W, Tomko Jr. RJ, Sakata E
Cell Rep 24 1301-1315.e5 (2018)
PMID: 30067984

Function and Biology Details

Sample name: 26S proteasome
Ligands: ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ADENOSINE-5'-TRIPHOSPHATE
Proteins: 26S proteasome, Proteasome subunit alpha type-1, Proteasome subunit alpha type-2, Proteasome subunit alpha type-3, Proteasome subunit alpha type-4, Proteasome subunit alpha type-5, Proteasome subunit alpha type-6, Probable proteasome subunit alpha type-7, Proteasome subunit beta type-1, Proteasome subunit beta type-2, Proteasome subunit beta type-3, Proteasome subunit beta type-4, Proteasome subunit beta type-5, Proteasome subunit beta type-6, Proteasome subunit beta type-7, 26S proteasome regulatory subunit RPN10, Ubiquitin carboxyl-terminal hydrolase RPN11, 26S proteasome regulatory subunit RPN12, 26S proteasome regulatory subunit RPN13, 26S proteasome complex subunit SEM1, 26S proteasome regulatory subunit RPN1, 26S proteasome regulatory subunit RPN2, 26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit RPN5, 26S proteasome regulatory subunit RPN6, 26S proteasome regulatory subunit RPN7, 26S proteasome regulatory subunit RPN8, 26S proteasome regulatory subunit RPN9, 26S proteasome regulatory subunit 7 homolog, 26S proteasome regulatory subunit 4 homolog, 26S proteasome regulatory subunit 6B homolog, 26S proteasome subunit RPT4, 26S proteasome regulatory subunit 6A, 26S proteasome regulatory subunit 8 homolog

Experimental Information Details

Resolution: 5.4Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)