EMDB EMD-4274

Single particle reconstruction
4.1Å resolution

CryoEM structure of E.coli RNA polymerase paused elongation complex without RNA hairpin bound to NusA

Map released:
Last modified:
Overview of EMD-4274
Source organisms:
Fitted atomic model: 6flp
Related EM entry by publication: EMD-4275
Primary publication:
Structural Basis for NusA Stabilized Transcriptional Pausing.
Guo X, Myasnikov AG, Chen J, Crucifix C, Papai G, Takacs M, Schultz P, Weixlbaumer A
Mol. Cell 69 816-827.e4 (2018)
PMID: 29499136

Function and Biology Details

Sample name: Cryo-EM structure of E. coli RNA polymerase paused elongation complex without RNA hairpin bound to NusA
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: DNA (30-MER), RNA (5'-R(P*GP*AP*UP*GP*UP*GP*UP*GP*CP*U)-3'), DNA (39-MER)
Proteins: Cryo-EM structure of E. coli RNA polymerase paused elongation complex without RNA hairpin bound to NusA, RNA polymerase and NusA, Nucleic acids, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega

Experimental Information Details

Resolution: 4.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)