EMDB EMD-4148

Single particle reconstruction
4.0Å resolution

Free monomeric RNA polymerase I at 4.0A resolution

Map released:
Last modified:
Overview of EMD-4148
Source organisms:
Fitted atomic model: 5m3m
Related EM entry by publication: EMD-4147, EMD-4149
Primary publication:
Structure of RNA polymerase I transcribing ribosomal DNA genes.
Neyer S, Kunz M, Geiss C, Hantsche M, Hodirnau VV, Seybert A, Engel C, Scheffer MP, Cramer P, Frangakis AS
Nature 540 607-610 (2016)
PMID: 27842382

Function and Biology Details

Sample name: Yeast RNA polymerase I elongation complex
Ligands: ZINC ION, SULFATE ION
Proteins: Yeast RNA polymerase I elongation complex, DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerase I subunit RPA135, DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA-directed RNA polymerases I, II, and III subunit RPABC2, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase I subunit RPA12, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, DNA-directed RNA polymerases I, II, and III subunit RPABC4, DNA-directed RNA polymerase I subunit RPA49, DNA-directed RNA polymerase I subunit RPA34, DNA-directed RNA polymerase I subunit RPA14, DNA-directed RNA polymerase I subunit RPA43

Experimental Information Details

Resolution: 4.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)