EMDB EMD-4088

Single particle reconstruction
4.9Å resolution

Monomeric RNA polymerase I at 4.9 A resolution

Map released:
Last modified:
Overview of EMD-4088
Source organisms:
Fitted atomic model: 5lmx
Related EM entry by publication: EMD-4086, EMD-4087
Primary publication:
The dynamic assembly of distinct RNA polymerase I complexes modulates rDNA transcription.
Torreira E, Louro JA, Pazos I, Gonzalez-Polo N, Gil-Carton D, Duran AG, Tosi S, Gallego O, Calvo O, Fernandez-Tornero C
Elife 6 - (2017)
PMID: 28262097

Function and Biology Details

Sample name: S. cerevisiae RNA polymerase I in the monomeric state
Ligand: ZINC ION
Proteins: S. cerevisiae RNA polymerase I in the monomeric state, DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerase I subunit RPA135, DNA-directed RNA polymerases I and III subunit RPAC1,DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerase I subunit RPA14, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA-directed RNA polymerases I, II, and III subunit RPABC2, DNA-directed RNA polymerase I subunit RPA43, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase I subunit RPA12, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, DNA-directed RNA polymerases I, II, and III subunit RPABC4, DNA-directed RNA polymerase I subunit RPA49, DNA-directed RNA polymerase I subunit RPA34

Experimental Information Details

Resolution: 4.9Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)