Happy Holidays and New year from the EMDB! Please be advised that the EMDB team will be operating at reduced capacity between December 15th - January 12th. With this in mind we would ask that you be prepared for longer reply times through both our helpdesk system and the OneDep deposition system. well done on a productive 2025, wishing you all the best and we will see you in 2026!

EMD-40862

Single-particle
2.7 Å
EMD-40862
3D View Gallery
Deposition: 24/05/2023
Map released: 27/12/2023
Last modified: 27/12/2023
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-40862
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 8sy5 summary report (pdf.gz) Map and 8sy5 FULL report (pdf.gz)
EMICSS annotations (xml)

E. coli DNA-directed RNA polymerase transcription elongation complex bound the unnatural dS-BTP base pair in the active site

EMD-40862

Single-particle
2.7 Å
EMD-40862
3D View Gallery
Deposition: 24/05/2023
Map released: 27/12/2023
Last modified: 27/12/2023
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Escherichia coli, synthetic construct
Sample: E. coli RNAP transcription elongation complex bound the unnatural dS-BTP base pair in the active site
Fitted models: 8sy5

Deposition Authors: Shan Z , Lyumkis D , Oh J , Wang D
A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E. coli RNA polymerase.
Oh J , Shan Z , Hoshika S , Xu J , Chong J, Benner SA, Lyumkis D , Wang D
(2023) Nat Commun , 14 , 8219 - 8219
PUBMED: 38086811
DOI: doi:10.1038/s41467-023-43735-9
ISSN: 2041-1723