EMDB EMD-4080

Single particle reconstruction
4.0Å resolution

Structure of bacterial 30S-IF3-mRNA-tRNA translation pre-initiation complex, closed form (state-4)

Map released:
Last modified:
Overview of EMD-4080
Source organisms:
Fitted atomic model: 5lmu
Primary publication:
Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation.
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell 167 133-144.e13 (2016)
PMID: 27662086

Function and Biology Details

Sample name: 30S-IF1-IF3-mRNA-tRNA pre-initiation complex (state-4)
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: 16S ribosomal RNA, mRNA, tRNAi
Proteins: 30S-IF1-IF3-mRNA-tRNA pre-initiation complex (state-4), 30S ribosomal protein S2, 30S ribosomal protein S3, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14 type Z, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, 30S ribosomal protein S18, 30S ribosomal protein S19, 30S ribosomal protein S20, 30S ribosomal protein Thx, Translation initiation factor IF-3

Experimental Information Details

Resolution: 4.0Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI POLARA 300
Detector: OTHER