EMDB EMD-3999

Single particle reconstruction
3.1Å resolution

Imidazoleglycerol-phosphate dehydratase

Map released:
Last modified:
Overview of EMD-3999
Source organism: Arabidopsis thaliana [3702]
Fitted atomic model: 6ezj
Related EM entry by publication: EMD-4160
Primary publication:
Elucidating the structural basis for differing enzyme inhibitor potency by cryo-EM.
Rawson S, Bisson C, Hurdiss DL, Fazal A, McPhillie MJ, Sedelnikova SE, Baker PJ, Rice DW, Muench SP
Proc. Natl. Acad. Sci. U.S.A. 115 1795-1800 (2018)
PMID: 29434040

Function and Biology Details

Sample name: Imidazoleglycerol-phosphate dehydratase (IGPD2) in complex with triazole-phosphonate inhibitor (C384)
Ligands: MANGANESE (II) ION, [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid
Proteins: Imidazoleglycerol-phosphate dehydratase (IGPD2) in complex with triazole-phosphonate inhibitor (C384), Imidazoleglycerol-phosphate dehydratase 2, chloroplastic

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: O
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)