EMDB EMD-3915

Subtomogram averaging
12.8Å resolution

Substrate processing state 26S proteasome (SPS2)

Map released:
Last modified:
Overview of EMD-3915
Source organism: Rattus norvegicus [10116]
Fitted atomic model: 6epe
Related EM entries by publication: EMD-3913, EMD-3914, EMD-3916, EMD-3917, EMD-4191
Primary publication:
In Situ Structure of Neuronal C9orf72 Poly-GA Aggregates Reveals Proteasome Recruitment.
Guo Q, Lehmer C, Martinez-Sanchez A, Rudack T, Beck F, Hartmann H, Perez-Berlanga M, Frottin F, Hipp MS, Hartl FU, Edbauer D, Baumeister W, Fernandez-Busnadiego R
Cell 172 696-705.e12 (2018)
PMID: 29398115

Function and Biology Details

Sample name: Substrate processing state 26S proteasome (SPS2)
Proteins: Substrate processing state 26S proteasome (SPS2), Proteasome subunit alpha type-6, Proteasome subunit alpha type-2, Proteasome subunit alpha type-4, Proteasome subunit alpha type-7, Proteasome subunit alpha type-5, Proteasome subunit alpha type-1, Proteasome subunit alpha type-3, Proteasome subunit beta type-6, Proteasome subunit beta type-7, Proteasome subunit beta type-3, Proteasome subunit beta type-2, Proteasome subunit beta type-5, Proteasome subunit beta type-1, Proteasome subunit beta type-4, 26S proteasome subunit S5a, Proteasome (Prosome, macropain) 26S subunit, non-ATPase, 14, Proteasome 26S subunit, non-ATPase 8, RCG28037, 26S proteasome non-ATPase regulatory subunit 2, 26S proteasome non-ATPase regulatory subunit 1, Proteasome (Prosome, macropain) 26S subunit, non-ATPase, 3, Proteasome (Prosome, macropain) 26S subunit, non-ATPase, 12, 26S proteasome non-ATPase regulatory subunit 11, Proteasome (Prosome, macropain) 26S subunit, non-ATPase, 6, Proteasome (Prosome, macropain) 26S subunit, non-ATPase, 7 (Predicted), 26S proteasome non-ATPase regulatory subunit 13, 26S proteasome regulatory subunit 7, 26S proteasome regulatory subunit 4, 26S proteasome regulatory subunit 6B, Proteasome 26S subunit, ATPase 6, 26S proteasome regulatory subunit 6A, 26S proteasome regulatory subunit 8

Experimental Information Details

Resolution: 12.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)