EMDB EMD-3847

Single particle reconstruction
3.2Å resolution

Cryo-EM structure of the 90S pre-ribosome from Chaetomium thermophilum

Map released:
Overview of EMD-3847
Fitted atomic model: 5oql
Primary publication:
3.2- Angstrom -resolution structure of the 90S preribosome before A1 pre-rRNA cleavage.
Cheng J, Kellner N, Berninghausen O, Hurt E, Beckmann R
Nat. Struct. Mol. Biol. - (2017)
PMID: 28967883

Function and Biology Details

Sample name: The 90S Pre-ribosomne
Ligand: ZINC ION
Nucleic acids: 35S rRNA, U3 snoRNA
Proteins: The 90S Pre-ribosomne, Periodic tryptophan protein 2-like protein, Utp2, Utp3, Utp4, Utp6, Utp7, Utp10, U3 small nucleolar RNA-associated protein 11, Utp12, Utp13, Utp14, Utp15, Utp17, Utp18, Putative U3 snoRNP protein, Utp24, Utp30, Nop1, Putative nucleolar protein, Nop58, Snu13, Rrp9, RNA 3'-terminal phosphate cyclase-like protein, Bms1, Imp3, Putative U3 small nucleolar ribonucleoprotein, Putative U3 small nucleolar ribonucleoprotein protein, Sof1, Emg1, KRR1 small subunit processome component, Pre-rRNA-processing protein PNO1, RNA cytidine acetyltransferase, Fcf2, 40S ribosomal protein S1, 40S ribosomal protein S4, 40S ribosomal protein s5-like protein, 40S ribosomal protein S6, 40S ribosomal protein S7-like protein, 40S ribosomal protein S8, 40S ribosomal protein s9-like protein, 40S ribosomal protein S13-like protein, 40S ribosomal protein S14-like protein, 40S ribosomal protein S16-like protein, 40S ribosomal protein S11-like protein, 40S ribosomal protein S22-like protein, 40S ribosomal protein s23-like protein, 40S ribosomal protein S24, 40S ribosomal protein S28-like protein, Faf1

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)