EMDB EMD-3836

Single particle reconstruction
3.6Å resolution

Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP

Map released:
Overview of EMD-3836
Source organism: Oryctolagus cuniculus [9986]
Fitted atomic model: 5ooc
Related EM entries by publication: EMD-3835, EMD-3837, EMD-3838, EMD-3839, EMD-4259
Primary publication:
Structural transitions of F-actin upon ATP hydrolysis at near-atomic resolution revealed by cryo-EM.
Merino F, Pospich S, Funk J, Wagner T, Kullmer F, Arndt HD, Bieling P, Raunser S
Nat. Struct. Mol. Biol. 25 528-537 (2018)
PMID: 29867215

Function and Biology Details

Sample name: Filamentous jasplakinolide-stabilized alpha actin in complex with ADP
Ligands: ADENOSINE-5'-DIPHOSPHATE, (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone, MAGNESIUM ION
Proteins: Filamentous jasplakinolide-stabilized alpha actin in complex with ADP, Actin, alpha skeletal muscle

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)