EMDB EMD-3777

Single particle reconstruction
4.6Å resolution

Cryo EM structure of the bacterial disaggregase ClpB (BAP form, DWB mutant), in the ATPgammaS state

Map released:
Last modified:
Overview of EMD-3777
Source organism: Escherichia coli [562]
Fitted atomic model: 5og1
Related EM entry by publication: EMD-3776
Primary publication:
Structural pathway of regulated substrate transfer and threading through an Hsp100 disaggregase.
Deville C, Carroni M, Franke KB, Topf M, Bukau B, Mogk A, Saibil HR
Sci Adv 3 e1701726-e1701726 (2017)
PMID: 28798962

Function and Biology Details

Sample name: ClpB homo hexamer, BAP form, double walker B mutant, in the ATPgammaS state
Proteins: ClpB homo hexamer, BAP form, double walker B mutant, in the ATPgammaS state, ClpB (BAP form, DWB mutant)

Experimental Information Details

Resolution: 4.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)