EMDB EMD-3770

Single particle reconstruction
4.2Å resolution

Human 40S-eIF2D-re-initiation complex

Map released:
Last modified:
Overview of EMD-3770
Source organisms:
Fitted atomic model: 5oa3
Primary publication:
Structural and Functional Insights into Human Re-initiation Complexes.
Weisser M, Schafer T, Leibundgut M, Bohringer D, Aylett CHS, Ban N
Mol. Cell 67 447-456.e7 (2017)
PMID: 28732596

Function and Biology Details

Sample name: human translation re-initiation complex of eIFD, initiator tRNA and the small ribosomal subunit
Ligand: ZINC ION
Nucleic acids: initiator Met-tRNA-i, 18S ribosomal RNA, IRES mRNA
Proteins: human translation re-initiation complex of eIFD, initiator tRNA and the small ribosomal subunit, eIF2D, small ribosomal subunit, HCV IRES mRNA, initiator Met-tRNA-i, Eukaryotic translation initiation factor 2D, 40S ribosomal protein SA, 40S ribosomal protein S3a, 40S ribosomal protein S2, 40S ribosomal protein S3, 40S ribosomal protein S4, X isoform, 40S ribosomal protein S5, 40S ribosomal protein S6, 40S ribosomal protein S7, 40S ribosomal protein S8, 40S ribosomal protein S9, 40S ribosomal protein S10, 40S ribosomal protein S11, 40S ribosomal protein S12, 40S ribosomal protein S13, 40S ribosomal protein S14, 40S ribosomal protein S15, 40S ribosomal protein S16, 40S ribosomal protein S17, 40S ribosomal protein S18, 40S ribosomal protein S19, 40S ribosomal protein S20, 40S ribosomal protein S21, 40S ribosomal protein S15a, 40S ribosomal protein S23, 40S ribosomal protein S24, 40S ribosomal protein S25, 40S ribosomal protein S26, 40S ribosomal protein S27, 40S ribosomal protein S28, 40S ribosomal protein S29, 40S ribosomal protein S30, Ribosomal protein S27a, Receptor of activated protein C kinase 1, RIBOSOMAL PROTEIN EL41

Experimental Information Details

Resolution: 4.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)