Single particle reconstruction
4.5Å resolution

Cryo-EM structure of a pre-catalytic human spliceosome primed for activation (B complex)

Map released:
Last modified:
Overview of EMD-3766
Source organism: Human [9606]
Fitted atomic model: 5o9z
Related EM entries by publication: EMD-3767, EMD-3768, EMD-3769
Primary publication:
Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, Luhrmann R, Stark H
Cell 170 701-713.e11 (2017)
PMID: 28781166

Function and Biology Details

Sample name: Human B Complex Spliceosome, sharpened map, masked refinement
Nucleic acids: MINX pre-mRNA, Human gene for small nuclear RNA U2 (snRNA U2), Homo sapiens U4A snRNA, Homo sapiens U5 A small nuclear RNA, Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA
Proteins: Human B Complex Spliceosome, sharpened map, masked refinement, Pre-mRNA-processing-splicing factor 8, 116 kDa U5 small nuclear ribonucleoprotein component, U5 small nuclear ribonucleoprotein 200 kDa helicase, U5 small nuclear ribonucleoprotein 40 kDa protein, U4/U6 small nuclear ribonucleoprotein Prp3, U4/U6 small nuclear ribonucleoprotein Prp4, Pre-mRNA-processing factor 6, U4/U6 small nuclear ribonucleoprotein Prp31, Pre-mRNA-splicing factor 38A, Thioredoxin-like protein 4A, Microfibrillar-associated protein 1, WD40 repeat-containing protein SMU1, Peptidyl-prolyl cis-trans isomerase H, Zinc finger matrin-type protein 2, NHP2-like protein 1, U4/U6.U5 tri-snRNP-associated protein 1, WW domain-binding protein 4, Protein Red, Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein G, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein-associated proteins B and B', Small nuclear ribonucleoprotein Sm D1, U6 snRNA-associated Sm-like protein LSm2, U6 snRNA-associated Sm-like protein LSm3, U6 snRNA-associated Sm-like protein LSm4, U6 snRNA-associated Sm-like protein LSm5, U6 snRNA-associated Sm-like protein LSm6, U6 snRNA-associated Sm-like protein LSm7, U6 snRNA-associated Sm-like protein LSm8, Splicing factor 3B subunit 1, Splicing factor 3B subunit 3 (SF3B3), Splicing factor 3B subunit 5, PHD finger-like domain-containing protein 5A, U2 small nuclear ribonucleoprotein A', U2 small nuclear ribonucleoprotein B''

Experimental Information Details

Resolution: 4.5Å
Resolution method: FSC 0.143 CUT-OFF
Detector: FEI FALCON III (4k x 4k)