EMDB EMD-3695

Single particle reconstruction
3.8Å resolution

Cryo-EM structure of RNA polymerase-sigma54 holo enzyme with promoter DNA closed complex

Map released:
Last modified:
Overview of EMD-3695
Source organisms:
Fitted atomic model: 5nsr
Related EM entry by publication: EMD-3696, EMD-3697
Primary publication:
Structures of RNA Polymerase Closed and Intermediate Complexes Reveal Mechanisms of DNA Opening and Transcription Initiation.
Glyde R, Ye F, Darbari VC, Zhang N, Buck M, Zhang X
Mol. Cell 67 106-116.e4 (2017)
PMID: 28579332

Function and Biology Details

Sample name: Cryo-EM structure of RNA polymerase-sigma54 holo enzyme with promoter DNA closed complex
Nucleic acids: Non-Template promoter DNA, Template DNA promoter
Proteins: Cryo-EM structure of RNA polymerase-sigma54 holo enzyme with promoter DNA closed complex, Escherichia coli core RNA polymerase, Sigma-54 transcription initiation factor, Sigma-54 promoter DNA, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor

Experimental Information Details

Resolution: 3.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)