EMDB EMD-3601

Single particle reconstruction
3.9Å resolution

Cryo EM structure of the conjugative relaxase TraI of the F/R1 plasmid system

Map released:
Last modified:
Overview of EMD-3601
Source organism: Escherichia coli [562]
Fitted atomic model: 5n8o
Primary publication:
Cryo-EM Structure of a Relaxase Reveals the Molecular Basis of DNA Unwinding during Bacterial Conjugation
Ilangovan A, Kay CWM, Roier S, El Mkami H, Salvadori E, Zechner EL, Zanetti G, Waksman G
Cell 169 708-721.e12 (2017)
PMID: 28457609

Function and Biology Details

Sample name: TraI-22mer complex
Nucleic acid: DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3')
Proteins: TraI-22mer complex, TraI protein, 22-mer DNA/RNA hybrid, DNA helicase I

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)