EMDB EMD-3589

Single particle reconstruction
4.2Å resolution

Cryo-EM structure of tsA201 cell alpha1B and betaI and betaIVb microtubules

Map released:
Last modified:
Overview of EMD-3589
Source organism: Homo sapiens [9606]
Fitted atomic model: 5n5n
Primary publication:
Tubulin isoform composition tunes microtubule dynamics.
Vemu A, Atherton J, Spector JO, Moores CA, Roll-Mecak A
Mol. Biol. Cell 28 3564-3572 (2017)
PMID: 29021343

Function and Biology Details

Sample name: Human tsA201 cell tubulin microtubules
Ligands: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER, MAGNESIUM ION, GUANOSINE-5'-TRIPHOSPHATE
Proteins: Human tsA201 cell tubulin microtubules, Tubulin beta chain, Tubulin alpha-1B chain

Experimental Information Details

Resolution: 4.2Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: FREALIGN
Microscope: FEI POLARA 300
Detector: DIRECT ELECTRON DE-20 (5k x 3k)