EMD-35421

Single-particle
2.92 Å
EMD-35421
3D View Gallery
Deposition: 18/02/2023
Map released: 17/01/2024
Last modified: 10/04/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-35421

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-8ifm summary report (pdf.gz) Map-8ifm FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of tetrameric SPARTA gRNA-ssDNA target complex in state 2

EMD-35421

Single-particle
2.92 Å
EMD-35421
3D View Gallery
Deposition: 18/02/2023
Map released: 17/01/2024
Last modified: 10/04/2024
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Thermoflavifilum thermophilum, Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Sample: CrtSPARTA tetramer complex in a second state
Fitted models: 8ifm

Deposition Authors: Zhang JT , Jia N , Huang HD
Target ssDNA activates the NADase activity of prokaryotic SPARTA immune system.
Zhang JT , Wei XY, Cui N, Tian R , Jia N
(2024) Nat Chem Biol , 20 , 503 - 511
PUBMED: 37932528
DOI: doi:10.1038/s41589-023-01479-z
ISSN: 1552-4469
Grant Support: