EMDB EMD-3542

Single particle reconstruction
4.6Å resolution

Structure of a spliceosome remodeled for exon ligation

Map released:
Last modified:
Overview of EMD-3542
Source organism: Baker's yeast [4932]
Related EM entry by publication: EMD-3539, EMD-3541
Primary publication:
Structure of a spliceosome remodelled for exon ligation.
Fica SM, Galej WP, Wilkinson ME, Oubridge C, Bai X-C, Newman AJ, Nagai K
Nature 542 377-380 (2017)
PMID: 28076345

Function and Biology Details

Sample name: Saccharomyces cerevisiae spliceosome. Complex C just after Prp16-mediated remodeling
Ligands: MAGNESIUM ION, POTASSIUM ION, INOSITOL HEXAKISPHOSPHATE, GUANOSINE-5'-TRIPHOSPHATE, ZINC ION
Nucleic acids: Intron of UBC4 pre-mRNA, 5'exon of UBC4 pre-mRNA, U2 snRNA, U6 snRNA, U5 snRNA, 3'exon of UBC4 pre-mRNA bound to Prp22 helicase
Proteins: Saccharomyces cerevisiae spliceosome. Complex C just after Prp16-mediated remodeling, Pre-mRNA-splicing factor 8, Pre-mRNA-splicing factor SNU114, Pre-mRNA-splicing factor CWC22, Pre-mRNA-splicing factor PRP46, Pre-mRNA-processing protein 45, Pre-mRNA-splicing factor BUD31, Pre-mRNA-splicing factor CEF1, Pre-mRNA-splicing factor CWC15, Pre-mRNA-splicing factor CWC21, Pre-mRNA-splicing factor CLF1, Pre-mRNA-splicing factor SYF1, Pre-mRNA-splicing factor 18, Pre-mRNA-splicing factor SLU7, Pre-mRNA-splicing factor SYF2, Small nuclear ribonucleoprotein-associated protein B, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein G, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, Pre-mRNA-splicing factor CWC2, Pre-mRNA-splicing factor SLT11, Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22, Pre-mRNA-processing factor 17, U2 small nuclear ribonucleoprotein A', U2 small nuclear ribonucleoprotein B'', Unknown protein

Experimental Information Details

Resolution: 4.6Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)