EMDB EMD-3536

Single particle reconstruction
7.8Å resolution

26S proteasome in presence of AMP-PNP (s3)

Map released:
Last modified:
Overview of EMD-3536
Source organism: Baker's yeast [559292]
Fitted atomic model: 5mpb
Related EM entries by publication: EMD-3534, EMD-3535, EMD-3537
Primary publication:
Structural insights into the functional cycle of the ATPase module of the 26S proteasome.
Wehmer M, Rudack T, Beck F, Aufderheide A, Pfeifer G, Plitzko JM, Forster F, Schulten K, Baumeister W, Sakata E
Proc. Natl. Acad. Sci. U.S.A. 114 1305-1310 (2017)
PMID: 28115689

Function and Biology Details

Sample name: 26S proteasome of Saccharomyces cerevisiae in presence of AMP-PNP
Ligands: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE
Proteins: 26S proteasome of Saccharomyces cerevisiae in presence of AMP-PNP, Proteasome subunit alpha type-1, Proteasome subunit alpha type-2, Proteasome subunit alpha type-3, Proteasome subunit alpha type-4, Proteasome subunit alpha type-5, Proteasome subunit alpha type-6, Probable proteasome subunit alpha type-7, Proteasome subunit beta type-1, Proteasome subunit beta type-2, Proteasome subunit beta type-3, Proteasome subunit beta type-4, Proteasome subunit beta type-5, Proteasome subunit beta type-6, Proteasome subunit beta type-7, 26S protease regulatory subunit 7 homolog, 26S protease regulatory subunit 4 homolog, 26S protease regulatory subunit 6B homolog, 26S protease subunit RPT4, 26S protease regulatory subunit 6A, 26S protease regulatory subunit 8 homolog, 26S proteasome regulatory subunit RPN10, Ubiquitin carboxyl-terminal hydrolase RPN11, 26S proteasome regulatory subunit RPN12, 26S proteasome regulatory subunit RPN13, 26S proteasome complex subunit SEM1, 26S proteasome regulatory subunit RPN1, 26S proteasome regulatory subunit RPN2, 26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit RPN5, 26S proteasome regulatory subunit RPN6, 26S proteasome regulatory subunit RPN7, 26S proteasome regulatory subunit RPN8, 26S proteasome regulatory subunit RPN9

Experimental Information Details

Resolution: 7.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)