EMDB EMD-3533

Single particle reconstruction
3.4Å resolution

Cryo-EM reconstruction of the 70S chloroplast ribosome

Map released:
Last modified:
Overview of EMD-3533
Source organism: Spinach [3562]
Fitted atomic model: 5mmm
Related EM entry by publication: EMD-3531, EMD-3532
Primary publication:
The complete structure of the chloroplast 70S ribosome in complex with translation factor pY.
Bieri P, Leibundgut M, Saurer M, Boehringer D, Ban N
EMBO J. 36 475-486 (2017)
PMID: 28007896

Function and Biology Details

Sample name: Chloroplast 70S ribosome
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: 23S ribosomal RNA, 5S ribosomal RNA, 4.5S ribosomal RNA, tRNA-Phe, 16S ribosomal RNA
Proteins: Chloroplast 70S ribosome, 50S ribosomal protein L31, 50S ribosomal protein L32, chloroplastic, 50S ribosomal protein L33, chloroplastic, 50S ribosomal protein L34, chloroplastic, 50S ribosomal protein L35, chloroplastic, 50S ribosomal protein L36, chloroplastic, plastid ribosomal protein cL37, PSRP5, 50S ribosomal protein 6, chloroplastic, 50S ribosomal protein L2, chloroplastic, plastid ribosomal protein uL3c, plastid ribosomal protein uL4c, plastid ribosomal protein uL5c, plastid ribosomal protein uL6c, plastid ribosomal protein bL9c, plastid ribosomal protein uL10c, 50S ribosomal protein L11, chloroplastic, 50S ribosomal protein L13, chloroplastic, 50S ribosomal protein L14, chloroplastic, plastid ribosomal protein uL15c, 50S ribosomal protein L16, chloroplastic, plastid ribosomal protein uL14c, plastid ribosomal protein uL18c, 50S ribosomal protein L19, chloroplastic, 50S ribosomal protein L20, chloroplastic, 50S ribosomal protein L21, chloroplastic, 50S ribosomal protein L22, chloroplastic, 50S ribosomal protein L23, chloroplastic, plastid ribosomal protein uL24c, plastid ribosomal protein bL27c, plastid ribosomal protein bL28c, plastid ribosomal protein uL29c, plastid ribosomal protein bS1c, 30S ribosomal protein S2, chloroplastic, 30S ribosomal protein S3, chloroplastic, 30S ribosomal protein S4, chloroplastic, 30S ribosomal protein S5, chloroplastic, plastid ribosomal protein bS6c, 30S ribosomal protein S7, chloroplastic, 30S ribosomal protein S8, chloroplastic, plastid ribosomal protein uS9c, plastid ribosomal protein uS10c, 30S ribosomal protein S11, chloroplastic, 30S ribosomal protein S12, chloroplastic, plastid ribosomal protein uS13c, 30S ribosomal protein S14, chloroplastic, 30S ribosomal protein S15, chloroplastic, 30S ribosomal protein S16, chloroplastic, plastid ribosomal protein uS17c, 30S ribosomal protein S18, chloroplastic, 30S ribosomal protein S19 alpha, chloroplastic, plastid ribosomal protein bS20c, plastid ribosomal protein bS21c, 30S ribosomal protein 2, chloroplastic, 30S ribosomal protein 3, chloroplastic, 30S ribosomal protein S31, chloroplastic, Ribosome-binding factor PSRP1, chloroplastic

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)