EMDB EMD-3532

Single particle reconstruction
3.6Å resolution

Cryo-EM reconstruction of the small subunit of the chloroplast ribosome

Map released:
Last modified:
Overview of EMD-3532
Source organism: Spinach [3562]
Fitted atomic model: 5mmj
Related EM entry by publication: EMD-3531, EMD-3533
Primary publication:
The complete structure of the chloroplast 70S ribosome in complex with translation factor pY.
Bieri P, Leibundgut M, Saurer M, Boehringer D, Ban N
EMBO J. 36 475-486 (2017)
PMID: 28007896

Function and Biology Details

Sample name: Chloroplast 70S ribosome
Ligand: MAGNESIUM ION
Nucleic acid: 16S ribosomal RNA
Proteins: Chloroplast 70S ribosome, 50S ribosomal protein L31, plastid ribosomal protein bS1c, 30S ribosomal protein S2, chloroplastic, 30S ribosomal protein S3, chloroplastic, 30S ribosomal protein S4, chloroplastic, 30S ribosomal protein S5, chloroplastic, plastid ribosomal protein bS6c, 30S ribosomal protein S7, chloroplastic, 30S ribosomal protein S8, chloroplastic, plastid ribosomal protein uS9c, plastid ribosomal protein uS10c, 30S ribosomal protein S11, chloroplastic, 30S ribosomal protein S12, chloroplastic, plastid ribosomal protein uS13c, 30S ribosomal protein S14, chloroplastic, 30S ribosomal protein S15, chloroplastic, 30S ribosomal protein S16, chloroplastic, plastid ribosomal protein uS17c, 30S ribosomal protein S18, chloroplastic, 30S ribosomal protein S19 alpha, chloroplastic, plastid ribosomal protein bS20c, plastid ribosomal protein bS21c, 30S ribosomal protein 2, chloroplastic, 30S ribosomal protein 3, chloroplastic, 30S ribosomal protein S31, chloroplastic, Ribosome-binding factor PSRP1, chloroplastic

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)