EMDB EMD-3531

Single particle reconstruction
3.2Å resolution

Cryo-EM reconstruction of the large subunit of the chloroplast ribosome

Map released:
Last modified:
Overview of EMD-3531
Source organism: Spinach [3562]
Fitted atomic model: 5mmi
Related EM entry by publication: EMD-3532, EMD-3533
Primary publication:
The complete structure of the chloroplast 70S ribosome in complex with translation factor pY.
Bieri P, Leibundgut M, Saurer M, Boehringer D, Ban N
EMBO J. 36 475-486 (2017)
PMID: 28007896

Function and Biology Details

Sample name: Chloroplast 70S ribosome
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: 23S ribosomal RNA, 5S ribosomal RNA, 4.5S ribosomal RNA, E-site tRNA
Proteins: Chloroplast 70S ribosome, 50S ribosomal protein L31, 50S ribosomal protein L32, chloroplastic, 50S ribosomal protein L33, chloroplastic, 50S ribosomal protein L34, chloroplastic, 50S ribosomal protein L35, chloroplastic, 50S ribosomal protein L36, chloroplastic, plastid ribosomal protein cL37, PSRP5, 50S ribosomal protein 6, chloroplastic, 50S ribosomal protein L2, chloroplastic, plastid ribosomal protein uL3c, plastid ribosomal protein uL4c, plastid ribosomal protein uL5c, plastid ribosomal protein uL6c, plastid ribosomal protein bL9c, plastid ribosomal protein uL10c, 50S ribosomal protein L11, chloroplastic, 50S ribosomal protein L13, chloroplastic, 50S ribosomal protein L14, chloroplastic, plastid ribosomal protein uL15c, 50S ribosomal protein L16, chloroplastic, plastid ribosomal protein bL17c, plastid ribosomal protein uL18c, 50S ribosomal protein L19, chloroplastic, 50S ribosomal protein L20, chloroplastic, 50S ribosomal protein L21, chloroplastic, 50S ribosomal protein L22, chloroplastic, 50S ribosomal protein L23, chloroplastic, plastid ribosomal protein uL24c, plastid ribosomal protein bL27c, plastid ribosomal protein bL28c, plastid ribosomal protein uL29c

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)