EMDB EMD-3525

Single particle reconstruction
3.6Å resolution

Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions

Map released:
Last modified:
Overview of EMD-3525
Source organism: Spinach [3562]
Fitted atomic model: 5mlc
Related EM entry by publication: EMD-3526
Primary publication:
Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions.
Graf M, Arenz S, Huter P, Donhofer A, Novacek J, Wilson DN
Nucleic Acids Res. 45 2887-2896 (2017)
PMID: 27986857

Function and Biology Details

Sample name: 50S subunit of the spinach chloroplast ribosome
Nucleic acids: 23S ribosomal RNA, chloroplastic, 5S ribosomal RNA, chloroplastic, 4.8S ribosomal RNA, chloroplastic
Proteins: 50S subunit of the spinach chloroplast ribosome, 50S ribosomal protein L2, chloroplastic, 50S ribosomal protein L3, chloroplastic, 50S ribosomal protein L4, chloroplastic, 50S ribosomal protein L5, chloroplastic, 50S ribosomal protein L6, chloroplastic, 50S ribosomal protein L9, chloroplastic, 50S ribosomal protein L13, chloroplastic, 50S ribosomal protein L14, chloroplastic, 50S ribosomal protein L15, chloroplastic, 50S ribosomal protein L16, chloroplastic, 50S ribosomal protein L17, chloroplastic, 50S ribosomal protein L18, chloroplastic, 50S ribosomal protein L19, chloroplastic, 50S ribosomal protein L20, chloroplastic, 50S ribosomal protein L21, chloroplastic, 50S ribosomal protein L22, chloroplastic, 50S ribosomal protein L23, chloroplastic, 50S ribosomal protein L24, chloroplastic, 50S ribosomal protein L27, chloroplastic, 50S ribosomal protein L28, chloroplastic, 50S ribosomal protein L29, chloroplastic, 50S ribosomal protein L32, chloroplastic, 50S ribosomal protein L33, chloroplastic, 50S ribosomal protein L34, chloroplastic, 50S ribosomal protein L35, chloroplastic, 50S ribosomal protein L36, chloroplastic, Ribosome-recycling factor, chloroplastic, PSRP5alpha, chloroplastic, PSRP6, chloroplastic

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: FREALIGN
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)