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EMD-35191

Single-particle
3.95 Å
EMD-35191
3D View Gallery
Deposition: 24/01/2023
Map released: 22/02/2023
Last modified: 03/07/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-35191
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 8i54 summary report (pdf.gz) Map and 8i54 FULL report (pdf.gz)
EMICSS annotations (xml)

Lb2Cas12a RNA DNA complex

EMD-35191

Single-particle
3.95 Å
EMD-35191
3D View Gallery
Deposition: 24/01/2023
Map released: 22/02/2023
Last modified: 03/07/2024
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Lachnospiraceae bacterium MA2020, synthetic construct
Sample: LbCas12a-crRNA-DNA ternary complex
Fitted models: 8i54

Deposition Authors: Li J, sivaraman J, Satoru M
Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage.
Jianwei L, Jobichen C , Machida S, Meng S, Read RJ, Hongying C, Jian S, Yuan YA, Sivaraman J
(2023) PLoS Biol , 21 , e3002023 - e3002023
PUBMED: 36917574
DOI: doi:10.1371/journal.pbio.3002023
ISSN: 1545-7885
Grant Support:
  • Ministry of Education (MoE, Singapore): R154-000-A39-112 (Singapore)
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