EMDB EMD-3460

Single particle reconstruction
3.8Å resolution

Cryo-EM structure of Lambda Phage protein GamS bound to RecBCD.

Map released:
Last modified:
Overview of EMD-3460
Source organisms:
Fitted atomic model: 5mbv
Primary publication:
Structural basis for the inhibition of RecBCD by Gam and its synergistic antibacterial effect with quinolones.
Wilkinson M, Troman L, Wan Nur Ismah WA, Chaban Y, Avison MB, Dillingham MS, Wigley DB
Elife 5 - (2016)
PMID: 28009252

Function and Biology Details

Sample name: RecBCD complex bound to inhibitory protein lambda GamS
Proteins: RecBCD complex bound to inhibitory protein lambda GamS, RecBCD helicase/nuclease complex, Lambda GamS, RecBCD enzyme subunit RecB, RecBCD enzyme subunit RecC, RecBCD enzyme subunit RecD, Host-nuclease inhibitor protein gam

Experimental Information Details

Resolution: 3.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)